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murine fibroblast cell line l929 (ATCC)

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ATCC murine fibroblast cell line l929
(a) Cytotoxicity analysis of <t>L929</t> cells after 24 h of exposure to NPs-Mg(OH) 2 -Alg at different concentrations. * p < 0.05 vs control; (b) cytotoxicity analysis of L929 cells after 24 h of exposure to NPs-CS-PPi at different concentrations. * p < 0.05 vs control.

(a) Cytotoxicity analysis of <t>L929</t> cells after 24 h of exposure to NPs-Mg(OH) 2 -Alg at different concentrations. * p < 0.05 vs control; (b) cytotoxicity analysis of L929 cells after 24 h of exposure to NPs-CS-PPi at different concentrations. * p < 0.05 vs control.

Murine Fibroblast Cell Line L929, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3285 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/murine+fibroblast+cell+line+l929/NCTC+clone+929/pmc13177163-42-1-6
Average 99 stars, based on 3285 article reviews

murine fibroblast cell line l929 - by Bioz Stars, 2026-11

99/100 stars

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1) Product Images from "Comparative Study of Chitosan-Pyrophosphate and Magnesium Hydroxide-Alginate Hybrid Nanoparticles: Physicochemical Properties and Cytocompatibility toward Vascular Calcification Applications"

Article Title: Comparative Study of Chitosan-Pyrophosphate and Magnesium Hydroxide-Alginate Hybrid Nanoparticles: Physicochemical Properties and Cytocompatibility toward Vascular Calcification Applications

Journal: ACS Omega

doi: 10.1021/acsomega.5c12883

(a) Cytotoxicity analysis of L929 cells after 24 h of exposure to NPs-Mg(OH) 2 -Alg at different concentrations. * p < 0.05 vs control; (b) cytotoxicity analysis of L929 cells after 24 h of exposure to NPs-CS-PPi at different concentrations. * p < 0.05 vs control.

Figure Legend Snippet: (a) Cytotoxicity analysis of L929 cells after 24 h of exposure to NPs-Mg(OH) 2 -Alg at different concentrations. * p < 0.05 vs control; (b) cytotoxicity analysis of L929 cells after 24 h of exposure to NPs-CS-PPi at different concentrations. * p < 0.05 vs control.

Techniques Used: Control

Cell Culture:

Article Title: Ferroptosis-activating metabolite acrolein antagonizes necroptosis and anti-cancer therapeutics.
Article Snippet: The human renal cancer cell lines A498 (KCLB, 30044) and Caki-1 (KCLB, 30046) were cultured in DMEM media supplemented with 1% nonessential amino acids, 1% penicillin and streptomycin, and 10% heatinactivated FBS. .. The murine fibroblast cell line L929 (ATCC, CCL-1) was cultured in Iscove’s modified Dulbecco’s medium (IMDM; Thermo Fischer Scientific, 12440053) supplemented with 10% FBS, 1% penicillin and streptomycin, and 1% non-essential amino acid. .. The human cervical cancer cell line HeLa (ATCC, CCL-2) was cultured in minimum essential medium Eagle (Sigma, M0643) supplemented with 1% penicillin and streptomycin, and 10% heatinactivated FBS.

Article Title: Boosting the Biodegradation and Bioactivity of PBS‐DLS Copolymers via Incorporation of PEG
Article Snippet: .. Dimethyl succinate (DMS, 98%, CAS: 106-65-0, molecular weight: 146.14 g mol−1) and poly(ethylene glycol) (PEG, CAS: 25 322-68-3, average molecular weight: 6000 g mol−1), murine fibroblast cell line (L929) (Collection No.: ATCC CCL 1), cell culture medium (Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), L-glutamine, penicillin-streptomycin, trypsin, and lipase from Pseudomonas cepacia (CAS Number: 9001-62-1) were purchased from Sigma-Aldrich (ul. ..

Article Title: Ferroptosis-activating metabolite acrolein antagonizes necroptosis and anti-cancer therapeutics
Article Snippet: The human renal cancer cell lines A498 (KCLB, 30044) and Caki-1 (KCLB, 30046) were cultured in DMEM media supplemented with 1% non-essential amino acids, 1% penicillin and streptomycin, and 10% heat-inactivated FBS. .. The murine fibroblast cell line L929 (ATCC, CCL-1) was cultured in Iscove’s modified Dulbecco’s medium (IMDM; Thermo Fischer Scientific, 12440053) supplemented with 10% FBS, 1% penicillin and streptomycin, and 1% non-essential amino acid. .. The human cervical cancer cell line HeLa (ATCC, CCL-2) was cultured in minimum essential medium Eagle (Sigma, M0643) supplemented with 1% penicillin and streptomycin, and 10% heat-inactivated FBS.

Modification:

Molecular Weight:

In Vitro:

Article Title: Safety evaluation of bacterial melanin as a plant growth stimulant for agricultural and food industry applications
Article Snippet: .. In Vitro Cytotoxicity Assay (MTT Assay) Cell Line and Culture Conditions: To assess cytotoxicity of BM in mammalian cells, the murine fibroblast cell line L929 (ATCC CCL-1) was used. ..

Cytotoxicity Assay:

MTT Assay:

other:

Article Title: Evaluation of 2,7-Naphthyridines as Targeted Anti-Staphylococcal Candidates with Microbiota-Sparing Properties
Article Snippet: Images were acquired at 20× magnification using Etaluma Lumascope 620 microscope (Etaluma Inc., San Diego, USA).

Article Title: Evaluation of 2,7-Naphthyridines as Targeted Anti-Staphylococcal Candidates with Microbiota-Sparing Properties.
Article Snippet: Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin and maintained at 37 ◦C in a humidified atmosphere of 5% CO2.

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Journal: ACS Omega

doi: 10.1021/acsomega.5c12883

Figure Lengend Snippet: (a) Cytotoxicity analysis of L929 cells after 24 h of exposure to NPs-Mg(OH) 2 -Alg at different concentrations. * p < 0.05 vs control; (b) cytotoxicity analysis of L929 cells after 24 h of exposure to NPs-CS-PPi at different concentrations. * p < 0.05 vs control.

Article Snippet: The murine fibroblast cell line L929 (ATCC NCTC clone 929 [L cell, CCL-1]) was used for cytotoxicity assays.

Techniques: Control

Biological functionality and biocompatibility of the PTPM hydrogel. a ) Tube formation assay in HUVECs, demonstrating the strongest proangiogenic activity in the PTPM hydrogel group. b , c ) Quantitative analyses of branch number and total tube length further confirmed its superior vascularization potential( n = 3 per group). d ) Intracellular ROS levels in L929 fibroblasts cultured with different hydrogels revealed the most efficient ROS scavenging in the PTPM group, e , f ) supported by quantitative fluorescence intensity and area measurements. g ) Scratch-wound assays at 0 and 24 h indicated markedly enhanced cell migration with PTPM treatment( n = 3 per group), h ) corroborated by statistical analysis of migration rates( n = 3 per group). i ) Live/dead fluorescence staining of L929 cells on days 1, 3 and 5 showed excellent cytocompatibility across all groups, with the PTPM hydrogel promoting the greatest proliferation over time. j–l ) Quantitative assessments of fluorescence intensity, viability, and proliferation on days 1, 3 and 5 further verified enhanced cellular growth and biocompatibility in the PTPM group( n = 3 per group). m–p ) qPCR analysis of proinflammatory (IL-1, iNOS) and anti-inflammatory markers (IL-4, CD206) demonstrated that the PTPM hydrogel most effectively suppressed inflammatory responses and promoted an anti-inflammatory phenotype( n = 3 per group). All data are presented as mean ± SD; * P < 0.05, ** P < 0.01, *** P < 0.001.HUVECs, human umbilical vein endothelial cells; ROS, reactive oxygen species; qPCR, quantitative polymerase chain reaction; NS, normal saline; PVA, polyvinyl alcohol; TSPBA, N¹-(4-boronobenzyl)-N³-(4-boronophenyl)-N¹,N¹,N³,N³-tetramethylpropane-1,3-diamine; PGS, Palygorskite; MoS₂, molybdenum disulfide; PT, PVA-TSPBA hydrogel; PTM, PVA-TSPBA@MoS₂ hydrogel; PTPM, PVA-TSPBA@PGS/MoS₂ hydrogel

Journal: Journal of Nanobiotechnology

Article Title: Injectable ROS-scavenging and NIR-responsive nanocomposite hydrogel for staphylococcus aureus-infected diabetic wound healing

doi: 10.1186/s12951-026-04306-4

Figure Lengend Snippet: Biological functionality and biocompatibility of the PTPM hydrogel. a ) Tube formation assay in HUVECs, demonstrating the strongest proangiogenic activity in the PTPM hydrogel group. b , c ) Quantitative analyses of branch number and total tube length further confirmed its superior vascularization potential( n = 3 per group). d ) Intracellular ROS levels in L929 fibroblasts cultured with different hydrogels revealed the most efficient ROS scavenging in the PTPM group, e , f ) supported by quantitative fluorescence intensity and area measurements. g ) Scratch-wound assays at 0 and 24 h indicated markedly enhanced cell migration with PTPM treatment( n = 3 per group), h ) corroborated by statistical analysis of migration rates( n = 3 per group). i ) Live/dead fluorescence staining of L929 cells on days 1, 3 and 5 showed excellent cytocompatibility across all groups, with the PTPM hydrogel promoting the greatest proliferation over time. j–l ) Quantitative assessments of fluorescence intensity, viability, and proliferation on days 1, 3 and 5 further verified enhanced cellular growth and biocompatibility in the PTPM group( n = 3 per group). m–p ) qPCR analysis of proinflammatory (IL-1, iNOS) and anti-inflammatory markers (IL-4, CD206) demonstrated that the PTPM hydrogel most effectively suppressed inflammatory responses and promoted an anti-inflammatory phenotype( n = 3 per group). All data are presented as mean ± SD; * P < 0.05, ** P < 0.01, *** P < 0.001.HUVECs, human umbilical vein endothelial cells; ROS, reactive oxygen species; qPCR, quantitative polymerase chain reaction; NS, normal saline; PVA, polyvinyl alcohol; TSPBA, N¹-(4-boronobenzyl)-N³-(4-boronophenyl)-N¹,N¹,N³,N³-tetramethylpropane-1,3-diamine; PGS, Palygorskite; MoS₂, molybdenum disulfide; PT, PVA-TSPBA hydrogel; PTM, PVA-TSPBA@MoS₂ hydrogel; PTPM, PVA-TSPBA@PGS/MoS₂ hydrogel

Article Snippet: The murine fibroblast cell line L929 (Cat. No. CL-0137) and fetal bovine serum (FBS, Cat. No. 164210) were obtained from Wuhan Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Tube Formation Assay, Activity Assay, Cell Culture, Fluorescence, Migration, Staining, Real-time Polymerase Chain Reaction, Saline

miRNA Binding efficiency and in vitro study on L929 cells. (A) Binding efficiency of miRNA‐loaded LNPs evaluated in different solvent conditions (methanol and water) and on different microfluidic channel material (PDMS and Glass); (B) Assessment of miRNA‐LNP integrity after sonification, heating, and combined sonification and heating (C) Cell proliferation analyzed by PrestoBlue assay after treatment with increasing doses of LNPs, free miRNA, and LNP‐miRNA complexes (red arrow indicates the volume selected for further studies) (n = 5 samples/group); (D) Percentage of live cells following treatment with miRNA, LNP, dialyzed LNP (LNP_dial), miRNA‐loaded LNP (LNP‐miRNA), and dialyzed miRNA‐loaded LNP (LNP‐miRNA_dial); (E) Quantification of total cell nuclei in each treatment group, including miRNA, LNP, LNP_dial, LNP‐miRNA, LNP‐miRNA_dial, and CTRL. For (E,F) Data represent mean ± SD of 9 analyzed fields from a single well (technical replicates).

Journal: Advanced Science

Article Title: Microfluidic‐Driven Lipid Nanoparticles for Improved miRNA Delivery via Endo‐Lysosomal Trafficking Optimization

doi: 10.1002/advs.202519225

Figure Lengend Snippet: miRNA Binding efficiency and in vitro study on L929 cells. (A) Binding efficiency of miRNA‐loaded LNPs evaluated in different solvent conditions (methanol and water) and on different microfluidic channel material (PDMS and Glass); (B) Assessment of miRNA‐LNP integrity after sonification, heating, and combined sonification and heating (C) Cell proliferation analyzed by PrestoBlue assay after treatment with increasing doses of LNPs, free miRNA, and LNP‐miRNA complexes (red arrow indicates the volume selected for further studies) (n = 5 samples/group); (D) Percentage of live cells following treatment with miRNA, LNP, dialyzed LNP (LNP_dial), miRNA‐loaded LNP (LNP‐miRNA), and dialyzed miRNA‐loaded LNP (LNP‐miRNA_dial); (E) Quantification of total cell nuclei in each treatment group, including miRNA, LNP, LNP_dial, LNP‐miRNA, LNP‐miRNA_dial, and CTRL. For (E,F) Data represent mean ± SD of 9 analyzed fields from a single well (technical replicates).

Article Snippet: L929 murine fibroblast cell line (cat. no. 400260) was obtained from Cytion.

Techniques: Binding Assay, In Vitro, Solvent, Prestoblue Assay

Time‐dependent intracellular localization of Cy3‐labeled miRNA relative to the cell nucleus in L929 fibroblasts. Representative confocal microscopy images showing Cy3‐labeled miRNA (yellow) and DAPI‐stained nuclei (blue) in L929 fibroblasts treated with free miRNA (A), LNP‐miRNA (B), or LNP‐miRNA_dial (C) at the indicated time points (30 min, 1 h, 2 h, 4 h, and 24 h). Cy3‐miRNA signal is predominantly cytoplasmic and localized in the perinuclear region, with formulation‐dependent differences in the timing and intensity of intracellular miRNA appearance. Scale bar: 50 µm.

Journal: Advanced Science

Article Title: Microfluidic‐Driven Lipid Nanoparticles for Improved miRNA Delivery via Endo‐Lysosomal Trafficking Optimization

doi: 10.1002/advs.202519225

Figure Lengend Snippet: Time‐dependent intracellular localization of Cy3‐labeled miRNA relative to the cell nucleus in L929 fibroblasts. Representative confocal microscopy images showing Cy3‐labeled miRNA (yellow) and DAPI‐stained nuclei (blue) in L929 fibroblasts treated with free miRNA (A), LNP‐miRNA (B), or LNP‐miRNA_dial (C) at the indicated time points (30 min, 1 h, 2 h, 4 h, and 24 h). Cy3‐miRNA signal is predominantly cytoplasmic and localized in the perinuclear region, with formulation‐dependent differences in the timing and intensity of intracellular miRNA appearance. Scale bar: 50 µm.

Article Snippet: L929 murine fibroblast cell line (cat. no. 400260) was obtained from Cytion.

Techniques: Labeling, Confocal Microscopy, Staining, Formulation

Endo‐lysosomal trafficking and intracellular redistribution of miRNA in L929 fibroblasts. (A) Schematic illustration of miRNA complexation with lipid nanoparticles (LNPs), cellular uptake via endocytosis, and subsequent trafficking through early and late endosomal and lysosomal compartments visualized using LysoTracker staining. (B) Representative confocal microscopy images of L929 fibroblasts treated with free miRNA, LNP‐miRNA, or dialyzed LNP‐miRNA_dial and imaged at 30 min, 1 h, 2 h, 4 h, and 24 h post‐treatment. Cy3‐labeled miRNA is shown in yellow, LysoTracker‐stained acidic compartments in red, and nuclei (DAPI) in blue. Scale bar: 50 µm.

Journal: Advanced Science

Article Title: Microfluidic‐Driven Lipid Nanoparticles for Improved miRNA Delivery via Endo‐Lysosomal Trafficking Optimization

doi: 10.1002/advs.202519225

Figure Lengend Snippet: Endo‐lysosomal trafficking and intracellular redistribution of miRNA in L929 fibroblasts. (A) Schematic illustration of miRNA complexation with lipid nanoparticles (LNPs), cellular uptake via endocytosis, and subsequent trafficking through early and late endosomal and lysosomal compartments visualized using LysoTracker staining. (B) Representative confocal microscopy images of L929 fibroblasts treated with free miRNA, LNP‐miRNA, or dialyzed LNP‐miRNA_dial and imaged at 30 min, 1 h, 2 h, 4 h, and 24 h post‐treatment. Cy3‐labeled miRNA is shown in yellow, LysoTracker‐stained acidic compartments in red, and nuclei (DAPI) in blue. Scale bar: 50 µm.

Article Snippet: L929 murine fibroblast cell line (cat. no. 400260) was obtained from Cytion.

Techniques: Staining, Confocal Microscopy, Labeling

Single‐cell correlation analysis of intracellular miRNA (Cy3, Alexa 546) and LysoTracker (Alexa 647) signal intensity in L929 fibroblasts treated with free miRNA (green), LNP‐miRNA (turquoise), or LNP‐miRNA_dial (blue) at different time points (30 min–24 h). Each dot represents an individual cell. Untreated L929 fibroblasts (red) are included as a control to define baseline LysoTracker signal and background miRNA fluorescence. The data represent measurements acquired from 16 fields of view per condition, collected from three independent samples per group.

Journal: Advanced Science

Article Title: Microfluidic‐Driven Lipid Nanoparticles for Improved miRNA Delivery via Endo‐Lysosomal Trafficking Optimization

doi: 10.1002/advs.202519225

Figure Lengend Snippet: Single‐cell correlation analysis of intracellular miRNA (Cy3, Alexa 546) and LysoTracker (Alexa 647) signal intensity in L929 fibroblasts treated with free miRNA (green), LNP‐miRNA (turquoise), or LNP‐miRNA_dial (blue) at different time points (30 min–24 h). Each dot represents an individual cell. Untreated L929 fibroblasts (red) are included as a control to define baseline LysoTracker signal and background miRNA fluorescence. The data represent measurements acquired from 16 fields of view per condition, collected from three independent samples per group.

Article Snippet: L929 murine fibroblast cell line (cat. no. 400260) was obtained from Cytion.

Techniques: Single Cell, Control, Fluorescence

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murine fibroblast cell line l929 (ATCC)

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1) Product Images from "Comparative Study of Chitosan-Pyrophosphate and Magnesium Hydroxide-Alginate Hybrid Nanoparticles: Physicochemical Properties and Cytocompatibility toward Vascular Calcification Applications"

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